![]() ![]() Best enzyme activity was observed in the presence of Mg2+ (specific activity= 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity= 1.02 U/mg protein). ![]() ![]() In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity= 5.24 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1 % glycerol (specific activity = 6.88 U/mg protein). The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. Very professional.This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis. Also did service work to thermo fisher microplate readers multiskan fc ex floroskan ascent spectrum flash and thermo microplate cell washers wellwash nunc versa wellwash ac wellwash 4 mk2 in our oxfordshire, cambridgeshire, surrey, london and hampshire offices. Had engineers from UK laboratory services come in to repair and calibrate our sanyo gallenkamp ovens + Forma centrafuge units and incubators, 3110 370, 371, 380, 381, 3307, 3308, 3310, 3311, TC 120, IR 230, steri-cycle steri-cult + Heraeus heracell 150i, 240i, BB6220, BBD6220, cytoperm water jacketed co2 incubators & MCO-17AIC, MCO-18AIC, MCO 19AIC, MCO 20AIC incubators, sanyo orbital incubator MIR-220u thermo herecell co2 incubators and a number of centrifuge units refrigerated sorvall t6000, RT7, Stratos Biofuge, Legend rt, st16, st 40, 5c-rc plus, legend rt, evolution rc, rc-5c, Thermo fisher scientific Heraeus primo, pico, fresco, RC3, RC5b, RC6, eco mt135, eppendorf micro centrifuge machine. Service and repair work to incubators, centrafuge units, microplate readers and microplate washer. ![]()
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